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Article

Analysis of the development of a morphological phenotype as a function of protein concentration in budding yeast.

Journal of visualized experiments : JoVE - 24 Mar 2010

Mukherjee Debarati, Sen Arpita, Aguilar R Claudio

Abstract excerpt

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae. Although this protocol is based on the overexpression of a protein, it can easily be adapted for morphological phenotypes dependent on...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 20336036
DOI: 10.3791/1863
PMCID: PMC3168202

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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