Article
Protease Do is essential for survival of Escherichia coli at high temperatures: its identity with the htrA gene product.
Biochemical and biophysical research communications - 30 Apr 1991
Seol J H, Woo S K, Jung E M, Yoo S J, Lee C S, Kim K J, Tanaka K, Ichihara A, Ha D B, Chung C H
Abstract excerpt
The DNA encoding protease Do was isolated from an E. coli genomic DNA library in lambda gt11, and cloned into a Bluescript plasmid. The cells transformed with the recombinant plasmid were able to overproduce protease Do and grew normally. A mutant lacking the protease activity was also isolated by interrupting the chromosomal DNA with the kan gene. The mutant showed a prolonged lag period and reduced ability to...
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