Article
Enhanced reverse transcription-PCR assay for detection of norovirus genogroup I.
Journal of clinical microbiology - 1 Aug 2006
Dreier Jens, Störmer Melanie, Mäde Dietrich, Burkhardt Sabine, Kleesiek Knut
Abstract excerpt
We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV GGI RT-PCR was determined by probit analysis with defined RNA...
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