Article
[Modified guanidine hydrochloride method for DNA extraction from cord blood used in HLA genotyping].
Zhongguo shi yan xue ye xue za zhi - 1 Apr 2006
Tang Xue-Wei, Liao Can, Li Yan, Xie Xing-Mei, Huang Yi-Ling
Abstract excerpt
To compare two different methods for extracting genomic DNA from cord blood and to evaluate their applications for HLA genotyping, the genomic DNA from 72 samples was extracted by guanidine hydrochloride (Gu * HCl) and modified guanidine hydrochloride, the DNA yield and purity were evaluated by spectrophotometry and detected by PCR with sequence-specific primers. The result showed that the genomic DNA was...
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