Article
A fibroblast-specific factor binds to an upstream negative control element in the promoter of the mouse alpha 1(I) collagen gene.
The Journal of biological chemistry - 25 Apr 1991
Ravazzolo R, Karsenty G, de Crombrugghe B
Abstract excerpt
We have identified, in nuclear extracts of NIH-3T3 fibroblasts, a factor which binds a sequence between -339 and -361 in the mouse alpha 1(I) collagen promoter. A 3-base pair substitution mutation introduced in this promoter element, which abolishes the binding of the factor, increases the activity of the promoter 4-fold in DNA transfection experiments. This suggests that this element is a negative regulator of...
Topics
- Animals
- Biological Factors
- Cell Line
- Collagen
- DNA
- DNA Fingerprinting
- Mice
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Sequence Homology, Nucleic Acid