Article
Characterization of the Escherichia coli K12 gltS glutamate permease gene.
Molecular & general genetics : MGG - 1 Mar 1991
Kalman M, Gentry D R, Cashel M
Abstract excerpt
The gltS gene is known to encode a sodium-dependent, glutamate-specific permease. We have localized the Escherichia coli K12 gltS gene with respect to the spoT gene, sequenced it, and recombined a null insertion-deletion allele into the chromosome without loss of viability. The gltS null allele gives a Glt- phenotype, i.e. it abolishes the ability of a gltCc host to grow on glutamate as sole carbon and nitrogen...
Topics
- Alleles
- Amino Acid Sequence
- Amino Acid Transport Systems, Acidic
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Frameshift Mutation
- Genes, Bacterial
- Genetic Complementation Test
- Genetic Linkage