Article
Rhizobium meliloti glutamate synthase: cloning and initial characterization of the glt locus.
Journal of bacteriology - 1 May 1990
Lewis T A, Gonzalez R, Botsford J L
Abstract excerpt
The genetic locus glt, encoding glutamate synthase from Rhizobium meliloti 1021, was selected from a pLAFR1 clone bank by complementation of the R. meliloti 41 Glt- mutant AK330. A fragment of cloned DNA complementing this mutant also served to complement the Escherichia coli glt null mutant PA340. Complementation studies using these mutants suggested that glutamate synthase expression requires two...
Topics
- Amino Acids
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Glutamate Synthase
- Kinetics
- Mutation
- Phenotype