Article
Comparison of polyclonal expansion methods to improve the recovery of cervical cytobrush-derived T cells from the female genital tract of HIV-infected women.
Journal of immunological methods - 31 Mar 2010
Bere Alfred, Denny Lynette, Hanekom Willem, Burgers Wendy A, Passmore Jo-Ann S
Abstract excerpt
Cervical cytobrushing is a useful and non-invasive method for obtaining mucosal mononuclear cells from the female genital tract, but yields few cells. The aim of this study was to compare in vitro expansion protocols (anti-CD3, anti-CD3/CD28 or Dynal anti-CD3/CD28 beads) and cytokine combinations...
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