Article
Microinjection of dsRNA into fully-grown mouse oocytes.
Cold Spring Harbor protocols - 1 Jan 2009
Stein Paula
Abstract excerpt
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing in a number of model systems. The following protocol describes delivery of a double-stranded RNA (dsRNA) of choice into fully-grown, germinal vesicle-intact (GV) mouse oocytes by microinjection....
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