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Article

Precise conditional immortalization of mouse cells using tetracycline-regulated SV40 large T-antigen.

Genesis (New York, N.Y. : 2000) - 1 Apr 2010

Anastassiadis Konstantinos, Rostovskaya Maria, Lubitz Sandra, Weidlich Stefanie, Stewart A Francis

Abstract excerpt

Cellular immortalization provides a way for expansion and subsequent molecular characterization of rare cell types. Ideally, immortalization can be achieved by the reversible expression of immortalizing proteins. Here, we describe the use of conditional immortalization based on a modified tetracycline-regulated system for the expression of SV40 large T-antigen in embryonic stem (ES) cells and mice. The modified...

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PMID: 20146354
DOI: 10.1002/dvg.20605

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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