Article
Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria.
FEBS letters - 11 Mar 1991
Quaade C, Hughes S D, Coats W S, Sestak A L, Iynedjian P B, Newgard C B
Abstract excerpt
Five variant transcripts of the single rat glucokinase gene have been described that are naturally expressed in islets of Langerhans, liver and anterior pituitary. Four of these were prepared as cDNA and expressed in bacteria in order to begin to address their physiological roles. Expression of constructs pGKB1 (normal islet/pituitary glucokinase) and pGKL1 (normal liver glucokinase) resulted in a...
Topics
- Animals
- Base Sequence
- Escherichia coli
- Gene Expression
- Genetic Variation
- Glucokinase
- Glucose
- Islets of Langerhans
- Isomerism
- Molecular Sequence Data
- Phosphorylation