Article
Cell biology assessment of glucokinase mutations V62M and G72R in pancreatic beta-cells: evidence for cellular instability of catalytic activity.
Diabetes - 1 Jul 2007
Arden Catherine, Trainer Alison, de la Iglesia Nuria, Scougall Kathleen T, Gloyn Anna L, Lange Alex J, Shaw James A M, Matschinsky Franz M, Agius Loranne
Abstract excerpt
Mutations in the glucokinase (GK) gene cause defects in blood glucose homeostasis. In some cases (V62M and G72R), the phenotype cannot be explained by altered enzyme kinetics or protein instability. We used transient and stable expression of green fluorescent protein (GFP) GK chimaeras in MIN6 beta-cells to study the phenotype defect of V62M and G72R. GK activity in lysates of MIN6 cell lines stably expressing...
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