Article
Simultaneous fluorescent monitoring of proteasomal subunit catalysis.
Journal of the American Chemical Society - 10 Feb 2010
Wakata Aya, Lee Hsien-Ming, Rommel Philipp, Toutchkine Alexei, Schmidt Marion, Lawrence David S
Abstract excerpt
The proteasome, a multicatalytic protease, displays distinct chymotrypsin-like, caspase-like, and trypsin-like activities at three different subunits of the multimeric complex. Fluorescent substrates for each of these active sites have been described. However, since the fluorescent properties of...
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