Article
Altering the binding activity and specificity of the leucine binding proteins of Escherichia coli.
The Journal of biological chemistry - 5 Apr 1991
Adams M D, Maguire D J, Oxender D L
Abstract excerpt
Two leucine-binding proteins with overlapping specificities for the branched-chain amino acids are present in Escherichia coli. In order to study the basis of specificity for the very similar hydrophobic ligands, we have constructed a series of site-directed mutants of both proteins based on inspection of the leucine-isoleucine-valine-binding protein crystal structure reported by Sack et al. (Sack, J. S., Saper,...
Topics
- Bacterial Proteins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Leucine
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Protein Conformation
- Substrate Specificity