Article
PCR amplification and genetic analysis in a microwell cell culturing chip.
Lab on a chip - 21 Dec 2009
Lindström Sara, Hammond Maria, Brismar Hjalmar, Andersson-Svahn Helene, Ahmadian Afshin
Abstract excerpt
We have previously described a microwell chip designed for high throughput, long-term single-cell culturing and clonal analysis in individual wells providing a controlled way of studying high numbers of individual adherent or non-adherent cells. Here we present a method for the genetic analysis of cells cultured on-chip by PCR and minisequencing, demonstrated using two human adherent cell lines: one wild type and...
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