Article
Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells.
British journal of haematology - 1 Feb 2010
Rice Claire M, Clavel Carlos, Mazo Manu, Prosper Felipe, Scolding Neil J
Abstract excerpt
This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media...
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