Article
CD45-CD14 +CD34 + murine bone marrow low-adherent mesenchymal primitive cells preserve multilineage differentiation potential in long-term in vitro culture.
Molecules and cells - 1 Jun 2011
Szade Krzysztof, Zuba-Surma Ewa, Rutkowski Andrzej J, Jozkowicz Alicja, Dulak Jozef
Abstract excerpt
Bone marrow-derived cells have been postulated as a source of multipotent mesenchymal stem cells (MSC). However, the whole fraction of MSC remains heterogeneous and the expansion of primitive subset of these cells is still not well established. Here, we optimized the protocol for propagating the low-adherent subfraction of MSC which results in long-term expansion of population characterized by...
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