Article
Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette.
Journal of Zhejiang University. Science. B - 1 Dec 2009
Liu Ming, Yang Jun, Cheng Yun-qing, An Li-jia
Abstract excerpt
Soybean transformation by ovary-drip was improved by optimizing the length of the transformation pathway by cutting the styles. These modifications facilitated soybean transformation manipulation and improved transformation reproducibility and efficiency. Using a linear minimal gus gene cassette as the foreign DNA, a maximum transformation frequency of 11% was obtained in flowers of the soybean cultivar 'Liaodou...
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