Article
Upstream sequence activation of Escherichia coli argT promoter in vivo and in vitro.
Biochemistry - 22 Jan 1991
Hsu L M, Giannini J K, Leung T W, Crosthwaite J C
Abstract excerpt
Escherichia coli argT promoter in a galK fusion construct is shown by BAL 31 deletion to require its upstream region for high in vivo activity. The extent of activation conferred by the upstream sequence from -130 to -38 is 25-fold. A spontaneous mutant containing a T to G transversion at -37 (i....
Topics
- Base Sequence
- Binding, Competitive
- DNA
- DNA-Directed RNA Polymerases
- Escherichia coli
- Galactokinase
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- RNA, Transfer, Arg
- Regulatory Sequences, Nucleic Acid
- Repetitive Sequences, Nucleic Acid
- Transcription, Genetic