Article
Upstream sequence activation of Escherichia coli argT promoter in vivo and in vitro.
Biochemistry - 22 Jan 1991
Hsu L M, Giannini J K, Leung T W, Crosthwaite J C
Abstract excerpt
Escherichia coli argT promoter in a galK fusion construct is shown by BAL 31 deletion to require its upstream region for high in vivo activity. The extent of activation conferred by the upstream sequence from -130 to -38 is 25-fold. A spontaneous mutant containing a T to G transversion at -37 (i.e., the T-37G promoter) shows a similar requirement; however, the upstream sequence producing a 10-fold effect spans...
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