Article
Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990
Weickert M J, Chambliss G H
Abstract excerpt
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many...
Topics
- Bacillus subtilis
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Enzyme Repression
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Operon
- Plasmids