Article
[Construction and application of a multiplex PCR system for genotyping of human red blood cell antigens].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Oct 2012
Gao Huan-huan, He Yun-lei, Ye Lu-yi, Wang Pan, Guo Zhong-hui, Zhu Zi-yan, Zhu Yong-ming
Abstract excerpt
OBJECTIVE: A reliable method for genotyping blood group antigens Dib, k, Jsb1910 and Jsb2019 was developed. Through screening for rare blood types, the National Rare Blood Bank of China may be enriched. METHODS: The controls for allele detection of blood groups Dib, k, Jsb1910 and Jsb2019 were prepared via polymerase chain reaction (PCR)-mediated gene site-directed mutagenesis (SDM) technique. Sequence-specific...
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