Article
Whole genome single nucleotide polymorphism based phylogeny of Francisella tularensis and its application to the development of a strain typing assay.
BMC microbiology - 7 Oct 2009
Pandya Gagan A, Holmes Michael H, Petersen Jeannine M, Pradhan Sonal, Karamycheva Svetlana A, Wolcott Mark J, Molins Claudia, Jones Marcus, Schriefer Martin E, Fleischmann Robert D, Peterson Scott N
Abstract excerpt
BACKGROUND: A low genetic diversity in Francisella tularensis has been documented. Current DNA based genotyping methods for typing F. tularensis offer a limited and varying degree of subspecies, clade and strain level discrimination power. Whole genome sequencing is the most accurate and reliable method to identify, type and determine phylogenetic relationships among strains of a species. However, lower cost...
Topics
- Bacterial Typing Techniques
- Cluster Analysis
- Comparative Genomic Hybridization
- Computational Biology
- DNA, Bacterial
- Francisella tularensis
- Genome, Bacterial
- Phylogeny
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA