Article
TaqMan real-time PCR assays for single-nucleotide polymorphisms which identify Francisella tularensis and its subspecies and subpopulations.
PloS one - 1 Jan 2014
Birdsell Dawn N, Vogler Amy J, Buchhagen Jordan, Clare Ashley, Kaufman Emily, Naumann Amber, Driebe Elizabeth, Wagner David M, Keim Paul S
Abstract excerpt
Francisella tularensis, the etiologic agent of tularemia and a Class A Select Agent, is divided into three subspecies and multiple subpopulations that differ in virulence and geographic distribution. Given these differences, there is a need to rapidly and accurately determine if a strain is F. tularensis and, if it is, assign it to subspecies and subpopulation. We designed TaqMan real-time PCR genotyping assays...
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