Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Validation-based insertional mutagenesis identifies lysine demethylase FBXL11 as a negative regulator of NFkappaB.

Proceedings of the National Academy of Sciences of the United States of America - 22 Sept 2009

Lu Tao, Jackson Mark W, Singhi Aatur D, Kandel Eugene S, Yang Maojing, Zhang Yi, Gudkov Andrei V, Stark George R

Abstract excerpt

We describe a highly efficient use of lentiviral validation-based insertional mutagenesis (VBIM) to generate large populations of mammalian cells in which a strong promoter is inserted into many different genomic loci, causing greatly increased expression of downstream sequences. Many different selections or screens can follow, to isolate dominant mutant clones with a desired phenotypic change. The inserted...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibiotics, Antineoplastic
Share to Bleomycin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to F-Box Proteins

Share to HT29 Cells

Share to Jumonji Domain-Containing Histone Demethylases

Share to Lysine

Share to Methylation

Share to Oxidoreductases, N-Demethylating

Source

Open in PubMed Open full text

Identifiers

PMID: 19805303
DOI: 10.1073/pnas.0908560106
PMCID: PMC2736141

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Validation-based insertional mutagenesis identifies lysine demethylase FBXL11 as a negative regulator of NFkappaB.PMID 19805303Read ↗

Mutagenesis by reversible promoter insertion to study the activation of NF-kappaB.Semantic proximity Read ↗

Validation-Based Insertional Mutagenesis (VBIM), A Powerful Forward Genetic Screening Strategy.Semantic proximity Read ↗

Biochemically deleterious human NFKB1 variants underlie an autosomal dominant form of common variable immunodeficiency.Semantic proximity Read ↗

Genome-wide siRNA screen for mediators of NF-κB activationSemantic proximity Read ↗

The NF1 gene contains hotspots for L1 endonuclease-dependent de novo insertion.Semantic proximity Read ↗

Fen1 mutations result in autoimmunity, chronic inflammation and cancers.Semantic proximity Read ↗

Role of lysine methylation of NF-κB in differential gene regulation.Semantic proximity Read ↗

A retroviral mutagenesis screen reveals strong cooperation between Bcl11a overexpression and loss of the Nf1 tumor suppressor geneSemantic proximity Read ↗

Insertional mutagenesis in mice deficient for p15Ink4b, p16Ink4a, p21Cip1, and p27Kip1 reveals cancer gene interactions and correlations with tumor phenotypes.Semantic proximity Read ↗

Improving transcriptional activity of human cytomegalovirus major immediate-early promoter by mutating NF-κB binding sites.Semantic proximity Read ↗

Mutational analysis reveals an essential role for the LXXLL motif in the transformation function of the human herpesvirus-8 oncoprotein, kaposin.Semantic proximity Read ↗

A comprehensive library of mutations of Epstein Barr virus.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.