Article
A comprehensive library of mutations of Epstein Barr virus.
The Journal of general virology - 1 Sept 2007
Chiu Ya-Fang, Tung Chao-Ping, Lee Yu-Hisu, Wang Wen-Hung, Li Ching, Hung Jia-Yan, Wang Chen-Yu, Kawaguchi Yasushi, Liu Shih-Tung
Abstract excerpt
A mutant library of 249 mutants with mutations that span the entire Epstein-Barr virus (EBV) genome was generated by transposition with EZ : : TN <KAN-2> and insertion with an apramycin resistance gene by a PCR-targeting method. This study also demonstrates the feasibility of generating deletions and site-specific mutations in the BRLF1 promoter on the EBV genome to determine the regions in the promoter that are...
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