Article
Following evolutionary paths to protein-protein interactions with high affinity and selectivity.
Nature structural & molecular biology - 1 Oct 2009
Levin Kalia Bernath, Dym Orly, Albeck Shira, Magdassi Shlomo, Keeble Anthony H, Kleanthous Colin, Tawfik Dan S
Abstract excerpt
How do intricate multi-residue features such as protein-protein interfaces evolve? To address this question, we evolved a new colicin-immunity binding interaction. We started with Im9, which inhibits its cognate DNase ColE9 at 10(-14) M affinity, and evolved it toward ColE7, which it inhibits promiscuously (Kd > 10(-8) M). Iterative rounds of random mutagenesis and selection toward higher affinity for ColE7, and...
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