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Article

Following evolutionary paths to protein-protein interactions with high affinity and selectivity.

Nature structural & molecular biology - 1 Oct 2009

Levin Kalia Bernath, Dym Orly, Albeck Shira, Magdassi Shlomo, Keeble Anthony H, Kleanthous Colin, Tawfik Dan S

Abstract excerpt

How do intricate multi-residue features such as protein-protein interfaces evolve? To address this question, we evolved a new colicin-immunity binding interaction. We started with Im9, which inhibits its cognate DNase ColE9 at 10(-14) M affinity, and evolved it toward ColE7, which it inhibits pro...

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Identifiers

PMID: 19749752
DOI: 10.1038/nsmb.1670

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19749752-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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