Article
Site-directed mutagenesis of arginine 246, glutamic acid 247, and histidine 248 in the eukaryotic transcription factor S-II.
The Journal of biological chemistry - 15 Jul 1990
Horikoshi N, Sekimizu K, Natori S
Abstract excerpt
When His248 of the transcription factor S-II was replaced by alanine or tyrosine, the activity of the resulting mutants was less than 30% of that of the wild-type S-II. When Arg246, Glu247, and His248 were all replaced by leucine, the resulting mutant showed complete loss of activity. These resul...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Base Sequence
- Chymotrypsin
- Cloning, Molecular
- Escherichia coli
- Glutamates
- Glutamic Acid
- Histidine
- Kinetics
- Leucine
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Peptide Fragments
- Plasmids
- RNA Polymerase II