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Article

A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry.

The FEBS journal - 1 Sept 2009

Diago-Navarro Elizabeth, Kamphuis Monique B, Boelens Rolf, Barendregt Arjan, Heck Albert J, van den Heuvel Robert H, Díaz-Orejas Ramón

Abstract excerpt

Kid, the toxin of the parD (kis, kid) maintenance system of plasmid R1, is an endoribonuclease that preferentially cleaves RNA at the 5' of A in the core sequence 5'-UA(A/C)-3'. A model of the Kid toxin interacting with the uncleavable mimetic 5'-AdUACA-3' is available. To evaluate this model, a significant collection of mutants in some of the key residues proposed to be involved in RNA binding (T46, A55, T69 and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19694809
DOI: 10.1111/j.1742-4658.2009.07199.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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