Article
Kis antitoxin couples plasmid R1 replication and parD (kis,kid) maintenance modules.
Plasmid - 1 Mar 2012
López-Villarejo Juan, Diago-Navarro Elizabeth, Hernández-Arriaga Ana María, Díaz-Orejas Ramón
Abstract excerpt
The coupling between the replication and parD (kis, kid) maintenance modules of R1 has been revisited here by the isolation of a significant collection of conditional replication mutants in the pKN1562 mini-R1 plasmid, and in its derivative, pJLV01, specifically affected in the RNase activity of the Kid toxin. This new analysis aims to identify key factors in this coupling. For this purpose we have quantified and...
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