Article
Analysis of immunoglobulin isotype blood levels, splenic B-cell phenotypes, and spleen cell immunoglobulin gene expression in mice infected with lactate dehydrogenase-elevating virus.
Viral immunology - 1 Jan 1990
Hovinen D E, Bradley D S, Cafruny W A
Abstract excerpt
B-lymphocyte activation was studied in mice infected with lactate dehydrogenase-elevating virus (LDV). ELISA determinations of blood total immunoglobulin levels demonstrated that, at 10 days post-infection (p.i.) with LDV, only the IgG2a isotype was elevated. DNA-excess dot-blot hybridization showed that RNA specific for IgG2a and IgA immunoglobulin isotypes was increased in the spleens of mice at 10 days p.i....
Topics
- Animals
- B-Lymphocytes
- Blotting, Western
- Cyclophosphamide
- Electrophoresis, Gel, Two-Dimensional
- Enzyme-Linked Immunosorbent Assay
- Female
- Genes, Immunoglobulin
- Hypergammaglobulinemia
- Immunoglobulin Isotypes