Article
A new approach for the evaluation of the human papillomavirus type 16 variability with high resolution melting analysis.
Journal of virological methods - 1 Dec 2009
Sabol Ivan, Cretnik Maja, Hadzisejdić Ita, Si-Mohamed Ali, Matovina Mihaela, Grahovac Blazenka, Levanat Sonja, Grce Magdalena
Abstract excerpt
Studies on the variability of human papillomavirus (HPV) type 16 are based mostly on DNA sequencing of the viral oncogenes E6 and E7. In order to simplify variant identification, high resolution melting (HRM) analysis, which has been shown to distinguish amplicons differing in a single nucleotide, was employed. Optimised HRM analysis was applied to 255 anogenital samples positive for HPV 16. The E6/E7 region of...
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