Article
Investigation of the methylation status around parent-of-origin detectable SNPs in imprinted genes.
Forensic science international. Genetics - 1 Sept 2009
Nakayashiki Nori, Takamiya Masataka, Shimamoto Kirito, Aoki Yasuhiro, Hashiyada Masaki
Abstract excerpt
Methylation of CpG dinucleotides was investigated in five regions by bisulphite treatment of gDNA, PCR and cloning/sequencing. The gDNA was prepared from peripheral blood, saliva, semen, nails and hair from the head. In gDNA from peripheral blood, three regions were investigated in 16, 23 and 24 individuals, respectively (Fig. 2). In gDNA from other sources, three or five regions were investigated in five...
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