Article
Instability of bacteriophage Mu transposase and the role of host Hfl protein.
Molecular microbiology - 1 Nov 1990
Gama M J, Toussaint A, Pato M L
Abstract excerpt
The activity of the transposase of bacteriophage Mu is unstable, requiring the protein to be synthesized throughout the lytic cycle (Pato and Reich, 1982). Using Western blot analysis, we analysed the stability of the transposase protein during the lytic cycle and found that it, too, is unstable. The instability of the protein is observed both in the presence and the absence of Mu DNA replication, and is...
Topics
- Bacteriophage mu
- DNA Replication
- Enzyme Stability
- Escherichia coli
- Genes, Viral
- Mutation
- Nucleotidyltransferases
- Plasmids
- Transposases
- Viral Proteins