Article
TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements.
Plant physiology - 1 Nov 2009
Perry Jillian, Brachmann Andreas, Welham Tracey, Binder Andreas, Charpentier Myriam, Groth Martin, Haage Kristina, Markmann Katharina, Wang Trevor L, Parniske Martin
Abstract excerpt
We have established tools for forward and reverse genetic analysis of the legume Lotus (Lotus japonicus). A structured population of M2 progeny of 4,904 ethyl methanesulfonate-mutagenized M1 embryos is available for single nucleotide polymorphism mutation detection, using a TILLING (for Targeting Induced Local Lesions IN Genomes) protocol. Scanning subsets of this population, we identified a mutation load of one...
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