Article
High-throughput and targeted genotyping of Lotus japonicus LORE1 insertion mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Urbański Dorian Fabian, Małolepszy Anna, Stougaard Jens, Andersen Stig Uggerhøj
Abstract excerpt
The Lotus Retrotransposon 1 (LORE1) is used for genome-wide mutagenesis of the model legume Lotus japonicus. Characterization of the LORE1 insertion sites in individual mutant lines is critical for development and use of the resource. Here we present guidelines for use of the LORE1 reverse genetics resource and provide detailed protocols for insertion site identification and validation. For high-throughput...
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