Article
Genome-wide LORE1 retrotransposon mutagenesis and high-throughput insertion detection in Lotus japonicus.
The Plant journal : for cell and molecular biology - 1 Feb 2012
Urbański Dorian Fabian, Małolepszy Anna, Stougaard Jens, Andersen Stig Uggerhøj
Abstract excerpt
Use of insertion mutants facilitates functional analysis of genes, but it has been difficult to identify a suitable mutagen and to establish large populations for reverse genetics in most plant species. The main challenge is developing efficient high-throughput procedures for both mutagenesis and identification of insertion sites. To date, only floral-dip T-DNA transformation of Arabidopsis has produced...
Topics
- Computational Biology
- DNA Primers
- Exons
- Genome, Plant
- Genotyping Techniques
- High-Throughput Screening Assays
- Lotus
- Mutagenesis, Insertional
- Mutation
- Retroelements
- Reverse Genetics