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Article

Nonfluorescent denaturing HPLC-based primer-extension method for allele-specific expression: application to analysis of mismatch repair genes.

Clinical chemistry - 1 Sept 2009

Aceto Gitana M, De Lellis Laura, Catalano Teresa, Veschi Serena, Radice Paolo, Di Iorio Angelo, Mariani-Costantini Renato, Cama Alessandro, Curia Maria Cristina

Abstract excerpt

BACKGROUND: Altered germline expression of genes may represent a powerful marker of genetic or epigenetic predisposition to cancer or other diseases. METHODS: We developed and validated a method of nonfluorescent primer extension that uses a single dideoxynucleotide and denaturing HPLC (DHPLC) to analyze the relative allele expression. We devised 5 independent assays for measuring allele-specific expression (ASE)...

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Topics

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Share to Adaptor Proteins, Signal Transducing
Share to Chromatography, High Pressure Liquid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Mismatch Repair

Share to Fluorescent Dyes

Share to MutL Protein Homolog 1

Share to MutS Homolog 2 Protein

Share to Nuclear Proteins

Share to Nucleic Acid Denaturation

Source

Identifiers

PMID: 19628660
DOI: 10.1373/clinchem.2009.126300

Genes

MLH1
MSH2

Provenance

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