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Article

Mutation analysis of MLH1 and MSH2 genes performed by denaturing high-performance liquid chromatography.

Journal of biochemical and biophysical methods - 4 Mar 2002

Kurzawski Grzegorz, Safranow Krzysztof, Suchy Janina, Chlubek Dariusz, Scott Rodney J, Lubiński Jan

Abstract excerpt

Mutation analysis of large genes, such as MSH2 and MLH1, is time-consuming and expensive. We investigated the sensitivity and specificity of DHPLC analysis for the detection of mutations within both MSH2 and MLH1. Studies included a series of 46 patients affected by colorectal cancer from HNPCC families. We confirmed 19 changes previously identified by DNA sequencing and, in a blind study, an additional 16 rare...

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Source

Identifiers

PMID: 11879922
DOI: 10.1016/s0165-022x(02)00003-9

Genes

MLH1
MSH2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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