Article
Similar conformers in the minus strands of divergent positive strand RNA phage.
Virology - 1 Dec 1991
Shaklee P N
Abstract excerpt
Minus strand RNAs of phages MS2 and Q beta, prepared by in vitro transcription, were each separated into two forms on nondenaturing agarose gels. Only a single form of RNA was observed for minus strands denatured with glyoxal prior to agarose analysis, demonstrating that the two forms were conformers in each case. Truncated and elongated versions of these RNAs, analyzed under nondenaturing conditions,...
Topics
- Chromatography, Agarose
- Deoxyribonucleases
- Genetic Variation
- Nucleic Acid Conformation
- Plasmids
- RNA Phages
- RNA, Viral
- Transcription, Genetic
- Virus Replication