[Construction of hp0523 gene mutant in Helicobacter pylori Cag-PAI and the influence on the ability of CagA protein translocation].

Abstract excerpt

OBJECTIVE: To construct the hp0523 gene mutant of Helicobacter pylori and investigate the function of hp0523 gene. METHODS: We designed and amplified the upstream homologous fragment and downstream homologous fragment of hp0523 gene via PCR method. We constructed the suicide plasmid pBlueKM40-deltahp0523 based on allelic exchange. We introduced the suicide plasmid pBlueKM40-deltahp0523 into Helicobacter pylori...

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