Article
Generation and identification of DNA sequence flanking T-DNA integration site of Trichoderma atroviride mutants with high dichlorvos-degrading capacity.
Bioresource technology - 1 Dec 2009
Sun Wenliang, Liu Lixing, Hu Xiaolu, Tang Jun, Liu Peng, Chen Jie, Chen Yunpeng
Abstract excerpt
A protocol for efficient Agrobacterium tumefaciens-mediated transformation (ATMT) of biocontrol fungus Trichoderma atroviride strain T23 was developed to construct mutants with improved dichlorvos-degradation ability. A transformation frequency of 5x10(-6) was achieved. Among 110 genetically stable T-DNA transformants of T. atroviride T23, two transformants, AMT-12 and AMT-28, confirmed by Southern blot analysis...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
