Degradation of cyanide by Trichoderma mutants constructed by restriction enzyme mediated integration (REMI).

doi:10.1016/j.biortech.2006.09.047

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Article

Degradation of cyanide by Trichoderma mutants constructed by restriction enzyme mediated integration (REMI).

Bioresource technology - 1 Nov 2007

Zhou Xiaoying, Xu Shufa, Liu Lixing, Chen Jie

Abstract excerpt

REMI technique was used to construct mutants with improved cyanide-degradation ability from biocontrol fungus Trichoderma koningii strain T30. The plasmid pV2 transformation was confirmed by PCR and Southern blot analysis. Out of 21 transformants, 15 single-copied transformants (71.4%) were found. To compare enzyme activities of rhodanese and cyanide hydratase, T. atroviride T23, T. harzianum T21 and their...

Topics

Base Sequence
Blotting, Southern
Cyanides
DNA Primers
Mutation
Polymerase Chain Reaction
Trichoderma

Source

Open in PubMed

Identifiers

PMID: 17112721
DOI: 10.1016/j.biortech.2006.09.047

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