Article
Degradation of cyanide by Trichoderma mutants constructed by restriction enzyme mediated integration (REMI).
Bioresource technology - 1 Nov 2007
Zhou Xiaoying, Xu Shufa, Liu Lixing, Chen Jie
Abstract excerpt
REMI technique was used to construct mutants with improved cyanide-degradation ability from biocontrol fungus Trichoderma koningii strain T30. The plasmid pV2 transformation was confirmed by PCR and Southern blot analysis. Out of 21 transformants, 15 single-copied transformants (71.4%) were found. To compare enzyme activities of rhodanese and cyanide hydratase, T. atroviride T23, T. harzianum T21 and their...
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