Detection of the common alpha-1-antitrypsin variants by denaturing gradient gel electrophoresis.

doi:10.1111/j.1469-1809.1991.tb00413.x

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Article

Detection of the common alpha-1-antitrypsin variants by denaturing gradient gel electrophoresis.

Annals of human genetics - 1 Jul 1991

Johnson P H, Cadiou H, Hopkinson D A

Abstract excerpt

The well-characterized and highly polymorphic human alpha-1-antitrypsin (AAT) gene was used as a test locus to evaluate the general applicability of denaturing gradient gel electrophoresis (DGGE) for the detection of single base change polymorphisms. We report the resolution of all the major alle...

Topics

Alleles
Base Sequence
DNA
Electrophoresis, Polyacrylamide Gel
Exons
Humans
Introns
Molecular Sequence Data
Nucleic Acid Denaturation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
alpha 1-Antitrypsin

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