PCR Detection of Enterohemorrhagic <i>Escherichia coli</i> O145 in Food by Targeting Genes in the <i>E. coli</i> O145 O-Antigen Gene Cluster and the Shiga Toxin 1 and Shiga Toxin 2 Genes

Abstract excerpt

Shiga toxin-producing Escherichia coli (STEC) strains belonging to serogroup O145 are an important cause of hemorrhagic colitis and hemolytic uremic syndrome worldwide. Cattle and other animals are potential reservoirs for this pathogen. To develop PCR assays for detection and identification of E. coli O145, the wzx (O-antigen flippase) and wzy (O-antigen polymerase) genes in the O145 O-antigen gene cluster that...

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