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Application of a Real‐Time PCR‐Based System for Monitoring of O26, O103, O111, O145 and O157 Shiga Toxin‐Producing Escherichia coli in Cattle at Slaughter

21 Feb 2012

Abstract excerpt

Faecal samples were collected from 573 slaughtered cattle aged between three and 24 months in seven abattoirs. After enrichment (mTSB with novobiocin), samples were screened by real-time PCR first for stx and if positive, tested for the top-five Shiga toxin-producing Escherichia coli (STEC) serogroups using PCR assays targeting genes specific for serogroups O26, O103, O111, O145 and O157. Of 563 samples with...

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PMID: 22348425
DOI: 10.1111/j.1863-2378.2012.01468.x

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