DNA sequence of the<i>Escherichia coli</i>O103 O antigen gene cluster and detection of enterohemorrhagic<i>E. coli</i>O103 by PCR amplification of the<i>wzx</i>and<i>wzy</i>genes

Abstract excerpt

Escherichia coli serogroup O103 has been associated with gastrointestinal illness and hemolytic uremic syndrome. To develop PCR-based methods for detection and identification of this serogroup, the DNA sequence of the 12,033-bp region containing the O antigen gene cluster of Escherichia coli O103 was determined. Of the 12 open reading frames identified, the E. coli O103 wzx (O antigen flippase) and wzy (O antigen...

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