Direct Detection and Characterization of Shiga Toxigenic <i>Escherichia coli</i> by Multiplex PCR for <i>stx</i> ₁ , <i>stx</i> ₂ , <i>eae</i> , <i>ehxA</i> , and <i>saa</i>

Abstract excerpt

We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strains capable of causing severe gastrointestinal and systemic diseases in humans. In this study, we...

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