Article
Development of a homologous transformation system for Aspergillus aculeatus based on the sC gene encoding ATP-sulfurylase.
Bioscience, biotechnology, and biochemistry - 1 May 2009
Adachi Hiromi, Tani Shuji, Kanamasa Shin, Sumitani Jun-ichi, Kawaguchi Takashi
Abstract excerpt
A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC(+) transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.
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