Article
Functional electric field changes in photoactivated proteins revealed by ultrafast Stark spectroscopy of the Trp residues.
Proceedings of the National Academy of Sciences of the United States of America - 12 May 2009
Léonard Jérémie, Portuondo-Campa Erwin, Cannizzo Andrea, van Mourik Frank, van der Zwan Gert, Tittor Jörg, Haacke Stefan, Chergui Majed
Abstract excerpt
Ultrafast transient absorption spectroscopy of wild-type bacteriorhodopsin (WT bR) and 2 tryptophan mutants (W86F and W182F) is performed with visible light excitation (pump) and UV probe. The aim is to investigate the photoinduced change in the charge distribution with 50-fs time resolution by probing the effects on the tryptophan absorption bands. A systematic, quantitative comparison of the transient...
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