Article
Structural events in the photocycle of green fluorescent protein.
The journal of physical chemistry. B - 25 Aug 2005
van Thor Jasper J, Zanetti Giulia, Ronayne Kate L, Towrie Michael
Abstract excerpt
Picosecond time-resolved mid-infrared absorption changes of the wild type green fluorescent protein from Aequorea victoria are reported on structural events during the photocycle. Concomitant with rapid H/D transfer following excitation of the neutral A state at 400 nm, a transient signal at 1721/1711 cm(-1) (H/D) developed belonging to protonated glutamate 222, which was definitively assigned using the E222D...
Topics
- Glutamine
- Green Fluorescent Proteins
- Kinetics
- Models, Molecular
- Mutation
- Photochemistry
- Protons
- Spectrometry, Fluorescence
